CLARITY for mapping the nervous system
Posted on Feb 03, 2013
Abstract
With potential relevance for brain-mapping work, hydrogel-based structures can now be built from within biological tissue to allow subsequent removal of lipids without mechanical disassembly of the tissue. this process creates a tissue-hydrogel hybrid that is physically stable, that preserves fine structure, proteins and nucleic acids, and that is permeable to both visible-spectrum photons and exogenous macromolecules. here we highlight relevant challenges and opportunities of this approach, especially with regard to integration with complementary methodologies for brain- mapping studies.
By Kwanghun Chung & Karl Deisseroth