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Dr. White Featured on Digital Pathology Place Podcast

Posted on Feb 17, 2022

Dr. Sharla White featured on Digital Pathology Place Podcast

Dr. White Featured on Digital Pathology Place Podcast

Dr. Sharla White was recently featured on the Digital Pathology Place Podcast, hosted by Dr. Aleksandra Żuraw.

In this episode, Drs. White and Żuraw discuss how tissue clearing and 3D immunofluorescence can take your tissue research to a whole new level. Even though tissues are tridimensional structures, most tissue research is done on two-dimensional tissue slides. This leaves a tremendous amount of biological information on the table. 

Immuno-oncology

With the rise of immuno-oncology, the importance of immune cell interactions within the tumor microenvironment is now routinely interrogated with immunofluorescent markers and the spatial relationships of different immune cell populations are investigated. But almost all researchers still use 2 dimensional slides. It’s hard to investigate what’s happening in a 3D space on a flat, two-dimensional tissue section  This is where tissue clearing and 3D immunofluorescence come into play.

CLARITY and 3D IHC

CLARITY tissue clearing technology, developed by ClearLight Bio, allows for maintaining the integrity of tissue and visualizing cells in their original place and shape at the same time by using 3D immunofluorescence.

In order to image deeper (beyond 100 micrometers), the light-scattering lipids of the tissue need to be removed and the refractive indexes of collagen, bone and other tissue components need to be aligned. This is done after fixing the tissue and embedding it in a hydrogel. It ensures that the tissue structure is maintained before the detergent is applied to wash out the light-scattering lipids.

Once tissue clearing is done, antibodies with properties and in amounts compatible with the process are used for 3D immunofluorescence.

Digital Pathology Place Podcast

Listen to this Digital Pathology Place podcast episode at the link below to hear the full conversation.

https://digitalpathologyplace.com/portfolio-item/how-tissue-clearing-based-3d-immunofluorescence-allows-for-seeing-more-biology-in-the-tissue-w-sharla-white-clearlight-biotechnologies/

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CLARITY Tissue Clearing Kits are for researchers who wish to perform lipid clearing of soft tissues on their own.

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