Optimized Antibodies

For CLARITY and Tru3D™

Review our growing public list of optimized antibodies or ask us about your target of interest.

See More Biology Using Antibodies and CLARITY

ClearLight Biotechnologies makes extensive use of antibodies for multiplexed immunofluorescence staining in 3D immunohistochemistry (3D IHC). Imaging antibodies in 3D reveals novel insights, unobtainable with other technology.

ClearLight has many optimized antibodies ready to use in your research. If you don’t see your antibody of interest on our list, we would love to talk with you. A small antibody feasibility study to test your antibody and tissue type with our methodology may be the next step.

Expertise in Thick Tissue Antibody Penetration

The CLARITY tissue clearing method works on thicker tissue samples compared to what is possible with the current gold standard FFPE method.

Fortunately, at ClearLight Biotechnologies we have expertise in proper antibody titration for thick tissue immunostaining penetration.
We have established a specific approach for immunostaining thicker tissues that results in uniform saturation and optimal antibody binding.

Improving the penetration rate of antibodies into thick tissues increases our ability to visualize cellular and subcellular structure. Our methodologies can achieve high signal-to-noise ratios enabling researchers to see more biology.

Optimized Antibodies for CLARITY

We have optimized the following antibodies for immunostaining mouse and human tissues. 

Human Tissues:

Optimized Antibodies for CLARITY and Human Tissue Type Immunostaining IHC
  • α-SMA
  • CD3
  • CD4
  • CD8
  • CD20
  • CD31
  • CD45
  • CD45RO
  • CD68
  • CD163
  • CK 8
  • EGFR
  • FAP alpha
  • FoxP3
  • HLA-DR
  • Ki67
  • Lectin
  • pan-cadherin
  • pan-cytokeratin
  • PD-1
  • PD-L1
  • PGP9.5
  • SOX10
  • Vimentin

Mouse Tissues:

Antibodies for CLARITY and Mouse Tissue Type Immunostaining IHC
  • α -SMA
  • Calbindin
  • CD3
  • CD8
  • CD31
  • CD45
  • CD163
  • CK 8
  • CK 19
  • F4/80
  • GFAP
  • Histone H3
  • Ki67
  • Lectin
  • MHC Class II
  • pan-cytokeratin
  • PGP9.5
  • phospho-Histone H3
  • PSD95

Don't See Your Antibody?

Antibody Selection and Feasibility

Sometimes researchers want to explore using an antibody that isn't in our optimized list. Customers choose their biomarkers of interest, and if ClearLight has an optimized antibody then we recommend it. Customers may also request that we use an optimized antibody that is commercially available. If at any point we have to optimize an antibody then we perform an antibody feasibility study. The first step is of course to contact us, and let us know more about your research and biomarkers of interest.

Optimized Antibodies for CLARITY FAQs

Services

It can be both, for a pilot it could be a new tissue or a new tissue and new antibodies. If at any point we have to optimize an antibody, then it becomes antibody feasibility.

We perform a concentration and titration assessment that evaluates the optimal concentration level of any given tissue preferably done with control tissues.

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It’s All About Expression

When you choose 3D IHC for your research, it’s important to find the right antibody and the right protocol that will address the questions you have. It all comes down to expression: a given antibody must express in your tissue sample with the signal strength and precision to present meaningful scientific results.

Other common immunostaining techniques, e.g., enzyme-linked immunosorbent assay (ELISA), western blot and chromogenic staining, while “tried and true,” can yield data that is muted and muddied and less precise than desired.

ClearLight Biotechnologies uses multiplexed immunofluorescence staining.
The benefits of multiplexed immunofluorescence staining:

  • Provides definitive antibody expression which is easy to visualize
  • Allows tissues to be stained multiple times with different sets of antibodies yielding nuanced information including relationships between biomarkers and cellular structure
  • Enables advanced methods of 3D tissue analysis, such as Tru3D™
Seeing Ahead with ClearLight Contract Research and Development Services for Pharmaceutical Companies

CLARITY Tissue Clearing

Do You Need a CLARITY Tissue Clearing Primer? Visit the CLARITY Tissue Clearing page to learn about tissue clearing and specific steps in the CLARITY tissue clearing process.

CLARITY Tissue Clearing

COVID-19 Researchers

CLARITY tissue clearing and 3D imaging help your COVID 19 research by showing you new mechanistic insights and bringing your vision into the cellular biome.

Our COVID 19 Resources

CLARITY Optimized Antibodies Download

Full resolution, printable 8" x 11" datasheet highlighting the current optimized antibodies for CLARITY from ClearLight Biotechnologies.

Creating Cures Community

We applaud the research community and their involvement in the drug discovery and development process. These are the modern heroes who will create the cures of tomorrow for the diseases that impact human potential and decrease lifespan. Perhaps you are one. Join us.

Previous Issues

Analysis can be performed for select biomarkers, please contact us to find out if your target of interest is one of them. Imaging is currently performed on select fields of view instead of the entire sample.

Submission Guidelines

Tissue Sample Requirements: