Human Lung Carcinoma: CLARITY Tissue Cleared - Immunostained - Imaged - DAPI (blue), pan-cadherin (green), CD8 (yellow), α-SMA (red)


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Leverage ClearLight for your Research

Work with Our Experts

ClearLight Biotechnologies provides expert-level tissue clearing using the CLARITY method, 3D immunostaining, 3D imaging (multiplexed fluorescence), and Tru3D® tissue analysis. Preclinical researchers who work with us from pharmaceutical, biotechnology and academic environments, gain a better understanding of the tissue and tumor microenvironment.

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Researchers See More Biology with ClearLight

See lung, tonsil, and brain tissues passively cleared using the CLARITY method followed by multiplex immunostaining. A multiplex is three biomarkers and a nuclear counterstain.

Mouse Brain: Passively cleared and immunostained normal mouse brain (5 mm dia. x 1 mm thick) that has been stained with DAPI to identify individual cells, Lectin-DyLight 594 to identify vasculature, and Histone H3-Alexa Fluor 647, a chromatin marker.

Lung Carcinoma: DAPI (blue), pan-cadherin (green), CD8 (yellow), α-SMA (red)

Human Tonsil: DAPI (blue), CD8 (yellow), pan-Cytokeratin (green), PD-L1 (red)

ClearLight Expertise

Our Genesis

ClearLight has worked with the CLARITY method since its founding in 2015 by Karl Deisseroth MD, PhD, professor of bioengineering and psychiatry at Stanford University. Dr. Deisseroth and colleagues invented and developed CLARITY lipid-clearing techniques originally to be applied to brain tissue.

Beyond the Brain

ClearLight has built upon the original CLARITY protocol, optimizing the reagents, developing expertise in thick tissue immunostaining penetration, and expanding beyond the brain to include organoids, eye, lymph nodes, skin, bone, kidney, liver, tonsil and other human and animal tissues.

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    Preclinical Drug Development

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    Published Antibodies

    Download our published list of antibodies for immunostaining mouse and human tissue. Don’t see your target of interest? No problem. Contact us about a pilot study.

    CLARITY Infographic

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    Analysis can be performed for select biomarkers, please contact us to find out if your target of interest is one of them. Imaging is currently performed on select fields of view instead of the entire sample.

    Submission Guidelines

    Tissue Sample Requirements: