IHC CRO Laboratory

3D IHC Services

ClearLight provides 3D IHC services to preclinical researchers who want to See More Biology in the tissue microenvironment. Imaging biomarkers in 3D reveals novel insights, unobtainable with traditional 2D techniques.

    We had tried several other methods prior to working with ClearLight, but the results were inconclusive and unsatisfactory. The video from the 3D IHC finally got us the confirmation that our experimental therapeutic may be working.
    Our investors were wowed by the 3D video [ClearLight provided]. They hadn’t seen anything like it and it clearly demonstrated that our research shows promise.
    Working with Sharla and the team helped us solidify our research goals.
    The images we received from ClearLight led to us revising our direction and cutting our losses on an approach that wasn’t working. In the end we estimate that insight saved us at least 1000 hours in staff time that could then be more profitably utilized.
    Thank you so much for all the work! We are greatly satisfied with the quality and responsiveness to our additional requests for processing, file sharing, etc. Great to work together.

    Why Choose ClearLight Bio for IHC Services?

    ClearLight provides 3D IHC services to preclinical researchers who want to See More Biology in the tissue microenvironment. Imaging biomarkers in 3D reveals novel insights, unobtainable with traditional 2D techniques. We use CLARITY tissue clearing followed by multiplexed immunohistochemistry and Tru3D image analysis.

    Working with ClearLight gives you access to expert-level CLARITY and 3D IHC Services:

    • Our founder, Karl Deisseroth, invented the CLARITY protocol
    • We are experts in thick tissue antibody immunostaining
    • We’ve expanded the CLARITY protocol beyond the brain
    • We are not your typical CRO

    CLARITY stands out from other tissue clearing protocols for three reasons:

    • Thick tissue antibody penetration allows for high-resolution 3D imaging of thick tissue samples with greater accuracy than is possible using other methods
    • Using CLARITY researchers have the potential to restain their samples multiple times, conserving precious tissue samples
    • High structural integrity is maintained within the tissue sample post-clearing, due to the hydrogel matrix

    For more information see our Tissue Clearing Comparison page.

    What is 3D IHC?

    ClearLight’s 3D IHC Services include:

    • Immunostaining deep into CLARITY-cleared tissue
    • Multiplexing with 3 immunostains + DAPI
    • Deep imaging using fluorescent microscopy
    • 3D image analysis of the tissue microenvironment

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      Company We Keep

      Preclinical researchers who work with ClearLight Biotechnologies are involved in pharmaceutical, biotechnology, and academic environments in the United States and around the world.

      See More Biology with 3D IHC Services

      We’ve performed CLARITY tissue clearing and 3D IHC on tissue types from brain to bone. Chances are, ClearLight has experience with the tissue types useful to your research.

      Human iPSC-derived Cortical Brain Organoids: DAPI (blue), tyrosine hydroxylase (teal/light blue), Phox2B (green), Doublecortin (red)

      Human Non-Small Cell Lung Carcinoma (NSCLC): DAPI (blue), pan-cadherin (green), CD8 (yellow), α-SMA (red)

      Human Tonsil: DAPI (blue), CD8 (yellow), pan-Cytokeratin (green), PD-L1 (red)

      Hep55 Syngeneic Mouse Tumor: DAPI (blue), pan-cytokeratin (green), CD163 (yellow), CD68 (red)

      Human Pancreatic Ductal Adenocarcinoma (PDAC): DAPI (blue), CD8 (red), pan-Cytokeratin (green), PD-L1 (yellow)

      Lung Carcinoma: DAPI (blue), pan-cadherin (green), CD8 (yellow), α-SMA (red)

      Human Tonsil: DAPI (blue), CD8 (yellow), pan-Cytokeratin (green), PD-L1 (red)

      Hepp-55 Syngeneic mouse tumor showing vascularization

      Stop Using a 2D Solution for a 3D Problem

      Elevate Research. Wow Colleagues. Outmaneuver Competition.

      Give Your Research the 3D Advantage. Contact ClearLight Today.

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        Analysis can be performed for select biomarkers, please contact us to find out if your target of interest is one of them. Imaging is currently performed on select fields of view instead of the entire sample.

        Submission Guidelines

        Tissue Sample Requirements: