True 3D Analysis in 3D Unlocks the Black Box of a Tissue to reveal the biological complexity
The Problem with 2D
Current gold standard techniques are over 100 years old and rely on the analysis of 2D thin section FFPE (~5-10 micron). Spatial and morphological analysis of the tissue microenvironment is highly limited as well as limitations with processing hundreds of slides. Therefore, researchers rely on a small sampling of the tissue to understand complex biological processes.
Cancer doesn’t limit itself to 2 dimensions.
Current technologies utilized for preclinical, diagnostic, prognostic, and predictive clinical cancer research are dependent upon two-dimensional (2D) analysis of formalin- fixed paraffin embedded (FFPE) tissue sections (5–10μm).[1]
It has become clear that the heterogeneity within the tumor microenvironment (TME) contributes significantly to the development and eventual metastasis of cancer, as well as the response and resistance to treatment.[2]
Moving Beyond Slices and Slides
What was science fiction a few years ago is today a reality: It is now possible to trace the path of a single nerve projection through a forest of other cells; Illuminate your tissue microenvironment in a 3D volume; See more biology in 3D; Process samples multiple times using new biomarkers without destroying the tissue; Automate this process and integrate it into your normal tissue processing work flow; Add digital spatial image analysis in 3D to facilitate pre-clinical and clinical research studies. The future has arrived.
The Solution
See More Biology
in True 3D
Researchers need to see more to explore further. Seeing whole-volume high-content imaging lets context into the tissue analysis phase. If drug discovery and research were a maze of dark paths ahead of you, this would be your flashlight, showing the path ahead and letting you avoid dead ends and see further.
Enter CLARITY
CLARITY is an acronym for
Clear
Lipid-exchanged
Acrylamide-hybridized
Rigid
Imaging / Immunostaining / in situ-hybridization-compatible
Tissue
hYdrogel)
CLARITY Method Overview
Tissues are embedded in a hydrogel matrix to create a mesh
Lipids are cleared to create an optically
transparent hybrid
Resultant hybrid is transparent to light and permeable to molecules
Molecular markers are added to highlight specific features
This is nondestructive processing and can be repeated multiple times
Image using Confocal, Light Sheet, Selective Plane Illumination Microscopy (SPIM)
The ClearLight Difference
Our imaging and informatics capabilities go far beyond immunostaining and tissue clearing. Our in-house proprietary AI and Machine-Learning processes aid in segmentation and classification of the sample at unprecedented detail.
ClearLight Biotechnologies
- Uses full volumetric imaging
- Complete spatial analysis in context
- Reveals relationships and how cells and stains relate to each other.
- High content imaging
- Obtaining high-resolution information while maintaining a global perspective
An Overview of the ClearLight Biotechnologies Proprietary 3D Software Workflow
Raw Data
Segmentation
Classification
Analysis
True 3D: The Next Dimension in Tissue Analysis
Disease is one thing that connects all of us, sadly. Every person reading this, others in the room, and the room beyond that, and the all rooms in all the cities, worldwide. Disease connects us all; it also destroys lives and reduces human potential. Thankfully researchers like you are involved in drug discovery and development.
If your research work will benefit from non-destructive tissue processing and True 3D image analysis let’s work together on new possibilities.