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3D Imaging

When researchers can view spatial relationships in X, Y, and Z dimensions the understanding of the tissue microenvironment can spark new discoveries. This is the capability we are developing at ClearLight Biotechnologies. Our Tissue Analysis begins with CLARITY Tissue Clearing followed by multiplexed immmunohistochemistry. The stained tissued is then placed in a refractive index matching mounting solution for imaging with a confocal or light sheet microscope or another imaging platform.

Researchers employ multiplex IHC staining to identify cell phenotypes of interest within the tissue microenvironment. Since the sample is embedded and intact within the hydrogel matrix the same tissue has the potential to be imaged entirely in three dimensions.
The tissue's transparency enables 3D imaging of subcellular components such as DNA, RNA, and proteins for analysis within the tumor microenvironment of a tissue.

For additional images and comparison to other protocols visit our Tissue Clearing Comparison page.
https://clearlightbiotechnologies.com/tissue-clearing-comparison/?utm_source=YouTube&utm_medium=video&utm_campaign=3DImaging

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For additional information or to download the CLARITY Tissue Clearing Infographic visit the links below:

Learn More About CLARITY Tissue Clearing
https://clearlightbiotechnologies.com/clarity-tissue-clearing/?utm_source=YouTube&utm_medium=video&utm_campaign=3DImaging

Download the CLARITY Tissue Clearing Infographic
https://clearlightbiotechnologies.com/clarity-tissue-clearing-infographic/?utm_source=YouTube&utm_medium=video&utm_campaign=3DImaging

Learn More About ClearLight Biotechnologies
https://clearlightbiotechnologies.com/?utm_source=YouTube&utm_medium=video&utm_campaign=3DImaging

For more detail about our services please visit the ClearLight Biotechnologies Services page.
https://clearlightbiotechnologies.com/services/?utm_source=YouTube&utm_medium=video&utm_campaign=3DImaging

On the services page you will also find:
Antibodies Download
CLARITY Infographic
Tru3D Download
ClearLight Biotechnologies Company Fact Sheet

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Other Videos to check out this YouTube Channel:
CLARITY 101 - https://youtu.be/q4JfjAoe4aQ
3D IHC - https://youtu.be/DoZ-JFgIB3U
3D Imaging - https://youtu.be/eyqnUo1UfJM
Why do 3D over 2D? - https://youtu.be/LYO0nK-MtoQ

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Still have questions?
If you still have questions about our services please visit our website. We are happy to receive your call, email, or answer your questions in online chat.
https://clearlightbiotechnologies.com/contact/?utm_source=YouTube&utm_medium=video&utm_campaign=3DImaging

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Connect:
Follow us on social media, signup for the Creating Cures Community Newsletter, and join the conversation.
https://clearlightbiotechnologies.com/connect/?utm_source=YouTube&utm_medium=video&utm_campaign=3DImaging

If you find this video helpful, please subscribe to this channel and click the notification bell to receive email notification as new videos are published.

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Why Choose 3D Imaging over Standard 2D?

Disease doesn't limit itself to two dimensions. Neither should you and your research. Biological tissues are complex and intrinsically three dimensional. When researchers can view spatial relationships in X, Y, and Z dimensions the understanding of the tissue microenvironment can spark new discoveries.

Current gold standard techniques are over 100 years old and rely on the analysis of 2D thin section FFPE. Spatial and morphological analysis of the tissue microenvironment is highly limited as well as the limitations associated with processing hundreds of slides. Therefore, researchers rely on a small sampling of the tissue to understand complex biological processes.

CLARITY based tissue clearing coupled with Tru3D analysis gives you 10 times more contiguous and more meaningful data as compared to current gold standard 2D FFPE thin sections.

With CLARITY and Tru3D imaging you get complete and contiguous rich data. With ClearLight Biotechnologies your team can See More Biology.

For additional images and comparison to other protocols visit our Tissue Clearing Comparison page.
https://clearlightbiotechnologies.com/tissue-clearing-comparison/?utm_source=YouTube&utm_medium=video&utm_campaign=3Dover2D

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For additional information or to download the CLARITY Tissue Clearing Infographic visit the links below:

Learn More About CLARITY Tissue Clearing
https://clearlightbiotechnologies.com/clarity-tissue-clearing/?utm_source=YouTube&utm_medium=video&utm_campaign=3Dover2D

Download the CLARITY Tissue Clearing Infographic
https://clearlightbiotechnologies.com/clarity-tissue-clearing-infographic/?utm_source=YouTube&utm_medium=video&utm_campaign=3Dover2D

Learn More About ClearLight Biotechnologies
https://clearlightbiotechnologies.com/?utm_source=YouTube&utm_medium=video&utm_campaign=3Dover2D

For more detail about our services please visit the ClearLight Biotechnologies Services page.
https://clearlightbiotechnologies.com/services/?utm_source=YouTube&utm_medium=video&utm_campaign=3Dover2D

On the services page you will also find:
Antibodies Download
CLARITY Infographic
Tru3D Download
ClearLight Biotechnologies Company Fact Sheet

----------
Other Videos to check out this YouTube Channel:
CLARITY 101 - https://youtu.be/q4JfjAoe4aQ
3D IHC - https://youtu.be/DoZ-JFgIB3U
3D Imaging - https://youtu.be/eyqnUo1UfJM
Why do 3D over 2D? - https://youtu.be/LYO0nK-MtoQ

----------
Still have questions?
If you still have questions about our services please visit our website. We are happy to receive your call, email, or answer your questions in online chat.
https://clearlightbiotechnologies.com/contact/?utm_source=YouTube&utm_medium=video&utm_campaign=3Dover2D

----------
Connect:
Follow us on social media, signup for the Creating Cures Community Newsletter, and join the conversation.
https://clearlightbiotechnologies.com/connect/?utm_source=YouTube&utm_medium=video&utm_campaign=3Dover2D

If you find this video helpful, please subscribe to this channel and click the notification bell to receive email notification as new videos are published.

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Introduction to ClearLight Biotechnologies 3D Immunohistochemistry

Imaging antibodies in 3D reveals novel insights unobtainable with other technology. We use CLARITY Tissue Clearing to render opaque tissues transparent. Once tissues are transparent they can be immunostained and imaged. CLARITY employs tissue lipid clearing prior to immunostaining. We have found that the work flow matters - Clear - Stain - Image. This prescribed order of operations produces superior results.

This introductory video shows numerous examples of 3D IHC.

For additional images and comparison to other protocols visit our Tissue Clearing Comparison page.
https://clearlightbiotechnologies.com/tissue-clearing-comparison/?utm_source=YouTube&utm_medium=video&utm_campaign=3DIHC

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For additional information or to download the CLARITY Tissue Clearing Infographic visit the links below:

Learn More About CLARITY Tissue Clearing
https://clearlightbiotechnologies.com/clarity-tissue-clearing/?utm_source=YouTube&utm_medium=video&utm_campaign=3DIHC

Download the CLARITY Tissue Clearing Infographic
https://clearlightbiotechnologies.com/clarity-tissue-clearing-infographic/?utm_source=YouTube&utm_medium=video&utm_campaign=3DIHC

Learn More About ClearLight Biotechnologies
https://clearlightbiotechnologies.com/?utm_source=YouTube&utm_medium=video&utm_campaign=3DIHC

For more detail about our services please visit the ClearLight Biotechnologies Services page.
https://clearlightbiotechnologies.com/services/?utm_source=YouTube&utm_medium=video&utm_campaign=3DIHC

On the services page you will also find:
Antibodies Download
CLARITY Infographic
Tru3D Download
ClearLight Biotechnologies Company Fact Sheet

----------
Other Videos to check out this YouTube Channel:
CLARITY 101 - https://youtu.be/q4JfjAoe4aQ
3D IHC - https://youtu.be/DoZ-JFgIB3U
3D Imaging - https://youtu.be/eyqnUo1UfJM
Why do 3D over 2D? - https://youtu.be/LYO0nK-MtoQ

----------
Still have questions?
If you still have questions about our services please visit our website. We are happy to receive your call, email, or answer your questions in online chat.
https://clearlightbiotechnologies.com/contact/?utm_source=YouTube&utm_medium=video&utm_campaign=3DIHC

----------
Connect:
Follow us on social media, signup for the Creating Cures Community Newsletter, and join the conversation.
https://clearlightbiotechnologies.com/connect/?utm_source=YouTube&utm_medium=video&utm_campaign=3DIHC

Introduction to CLARITY Tissue Clearing method. For additional information or to download the CLARITY Tissue Clearing Infographic visit the links below:

Learn More About CLARITY Tissue Clearing
https://clearlightbiotechnologies.com/clarity-tissue-clearing/?utm_source=YouTube&utm_medium=video&utm_campaign=CLARITY101

Download the CLARITY Tissue Clearing Infographic
https://clearlightbiotechnologies.com/clarity-tissue-clearing-infographic/?utm_source=YouTube&utm_medium=video&utm_campaign=CLARITY101

See the Tissue Clearing Comparison
https://clearlightbiotechnologies.com/tissue-clearing-comparison/?utm_source=YouTube&utm_medium=video&utm_campaign=CLARITY101

Learn More About ClearLight Biotechnologies
https://clearlightbiotechnologies.com/?utm_source=YouTube&utm_medium=video&utm_campaign=CLARITY101

The human metastatic lymph node associated with a Stage 2A non-small cell lung cancer adenosquamous tumor. Pan-cadherin, a cell adhesion marker, was used to observe tumor cells, while CD8 denoted the cytotoxic T cells, and alpha smooth muscle actin (α-SMA) was used as a marker for stromal cells. DAPI was used as a nuclear counterstain.

See more biology with ClearLight Biotechnologies.

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about CLARITY and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about CLARITY and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/

ClearLight is working on the next dimension in tissue analysis in 3d. Here we present a 3D model used to segment and classify cells in a tonsil.

First, DAPI stains all the cells in our tissues. Then our AI method can automatically process this information and represents each cell nuclei with a different color. You can see how PDL1 is used to automatically discriminate which of the cells are PDL1 positive, and display them separately. You can then see PanCK, which stains epithelial cells and then finally you see the CD8 marker.

Altogether, this AI method provides the information required for a thorough characterization of the spatial distributions, morphological properties of all the cells within the imaged sample, as well as the classification of different cell types revealed by the biomarkers employed.

TRUE 3D Spatial Analysis in 3D - The next dimension in tissue analysis

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about CLARITY and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/

Using artificial intelligence and machine learning techniques, algorithms were created to extract data from either confocal or light sheet microscope imaging and perform accurate cell segmentation using DAPI as a feature extraction marker in human breast cancer samples prepared by the CLARITY tissue clearing technique.

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about CLARITY and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about CLARITY and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about CLARITY and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/