Videos - ClearLight Biotechnologies

Human NSCLC associated metastatic lymph node Cleared and Immustained with IHC

The human metastatic lymph node associated with a Stage 2A non-small cell lung cancer adenosquamous tumor. Pan-cadherin, a cell adhesion marker, was used to observe tumor cells, while CD8 denoted the cytotoxic T cells, and alpha smooth muscle actin (α-SMA) was used as a marker for stromal cells. DAPI was used as a nuclear counterstain.

See more biology with ClearLight Biotechnologies.

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about Clarity and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/
Enter the MicroEnvironment with ClearLight Biotechnologies

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about Clarity and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/
Segmentation and classification of tonsil tissue prepared by the CLARITY method

ClearLight is working on the next dimension in tissue analysis in 3d. Here we present a 3D model used to segment and classify cells in a tonsil.

First, DAPI stains all the cells in our tissues. Then our AI method can automatically process this information and represents each cell nuclei with a different color. You can see how PDL1 is used to automatically discriminate which of the cells are PDL1 positive, and display them separately. You can then see PanCK, which stains epithelial cells and then finally you see the CD8 marker.

Altogether, this AI method provides the information required for a thorough characterization of the spatial distributions, morphological properties of all the cells within the imaged sample, as well as the classification of different cell types revealed by the biomarkers employed.

TRUE 3D Spatial Analysis in 3D - The next dimension in tissue analysis

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about Clarity and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/
Clarity Process Machine Learning / AI

Using artificial intelligence and machine learning techniques, algorithms were created to extract data from either confocal or light sheet microscope imaging and perform accurate cell segmentation using DAPI as a feature extraction marker in human breast cancer samples prepared by the CLARITY tissue clearing technique.

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about Clarity and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/
Mouse kidney

Normal mouse kidney tissue was fixed and embedded in a hydrogel matrix and lipid cleared using the CLARITY tissue processing method. The cleared tissue was stained with an antibody against alpha smooth muscle actin and counterstained using DAPI. The sample was imaged to a depth of 800 microns using a Leica SP8 confocal and a 10x long working distance objective and imaged using IMARIS software.

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about Clarity and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/
Human Lung cancer

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about Clarity and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/
Human Lung cancer 5

A frozen human stage IB squamous cell carcinoma lung cancer tissue was fixed and embedded in a hydrogel matrix and lipid cleared using the CLARITY method and imaged using a Leica SP8 confocal microscope and a long working distance 5x objective. Tissue autofluorescence demonstrates intact tissue morphology of this lung cancer specimen.
Human Lung Cancer 4

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about Clarity and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/
Human Lung cancer 3

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about Clarity and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/
Human Lung cancer 2

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about Clarity and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/
Human Breast cancer

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about Clarity and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/
Human Breast cancer 2

CLARITY is easy to adopt, tailored to your lab work, and provides more data for less. ClearLight Biotechnologies is eager to answer your questions about Clarity and Tru3D - allowing you to enter the tumor microenvironment for unprecedented perspectives on the biological spatial relationships.

Learn more about ClearLight Biotechnologies Services: https://clearlightbiotechnologies.com/services/

Learn more about ClearLight Biotechnologies Technology: https://clearlightbiotechnologies.com/technology/

Learn More about CLARITY Tissue Clearing:
https://clearlightbiotechnologies.com/clarity-tissue-clearing/

Download the Free CLARITY Tissue Clearing Infographic: https://clearlightbiotechnologies.com/CLARITY-Tissue-Clearing-Infographic

Learn More about CLARITY IHC Multiplexing with CLARITY and Tru3D https://clearlightbiotechnologies.com/immunohistochemistry/

Analysis can be performed for select biomarkers, please contact us to find out if your target of interest is one of them. Imaging is currently performed on select fields of view instead of the entire sample.

Submission Guidelines

The submitted sample must be pre-fixed in either
- 4% paraformaldehyde (PFA) or
- 10% neutral buffered formalin (NBF) for 24 hours.
  - Unfixed samples will not be accepted for lab services.
  - Samples prepared with fixatives other than 4% PFA or 10% NBF will not be accepted and have not been validated with our protocol.
- The tissue must be noted:
  - perfused or not perfused
- All submitted samples must meet the noted specifications listed in Tissue Sample Requirement.
  - Samples that do not meet the size, species, or tissue sample type will not be accepted.
- Samples must be shipped in a sealed container (pick 1)
  - PBS or TBS solution.
- Shipped samples should be scheduled for delivery to ClearLight Biotechnologies Monday through Wednesday with a provided tracking number.
  - Shipping company
  - Tracking number

Tissue Sample Requirements:

- Sample Size dimensions:
  - 5 mm dia. x 1 mm thick or
  - 5 x 5 x 1 mm
- Acceptable Sample Species:
  - Mouse
  - Human
- Acceptable Tissue type:
  - Tumor
    - Syngeneic
    - Xenograft
    - PDX (patient derived xenograft)
    - Primary
  - Kidney
  - Spleen
  - Lung
  - Tonsil
  - Brain
  - Pancreas
  - Liver
  - Mammary

Video Gallery

Human NSCLC associated metastatic lymph node Cleared and Immustained with IHC

Enter the MicroEnvironment with ClearLight Biotechnologies

Segmentation and classification of tonsil tissue prepared by the CLARITY method

Clarity Process Machine Learning / AI

Mouse kidney

Human Lung cancer

Human Lung cancer 5

Human Lung Cancer 4

Human Lung cancer 3

Human Lung cancer 2

Human Breast cancer

Human Breast cancer 2

See More Biology and Explore Further.

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Service Requirements:

(800) 251-8905

Submission Guidelines

Tissue Sample Requirements: