3D IHC Multiplexing

with CLARITY and Tru3D

 

3D Immunohistochemistry

Modern medicine is filled with technology, specialization, and science that builds upon earlier work. Like other science and technology, immunohistochemistry (IHC) has now been assimilated into the general body of medical knowledge. IHC is now an indispensable tool for the diagnosis, prognosis and treatment of disease as well as being a foundational tool for in biomedical research and development around the world. That wasn’t always the case. IHC was built on the shoulders of giants, one scientific giant in particular, Dr. Albert Coons.

3D IHC Inspiration

The very foundation of Immunohistochemistry hearkens back to 1939 when Dr. Albert Coons was on vacation in Berlin, Germany. Dr. Coons was pondering ways to locate and identify antibodies as part of his study of rheumatic fever. On that fateful trip he gained the insight that he could put a visible label on an antibody molecule. This would allow targeted proteins to be seen within a diseased or normal tissue using a light microscope. He envisioned they would become brightly fluorescent. The concept was eventually born out and the idea took hold. Other researchers in laboratories around the world began using this method and the technique of immunofluorescence flourished.

CLARITY Processed and IHC Stained Tissue Example

Innovation Follows Discovery

Most science and technology breakthroughs are followed by additional innovations after the initial discovery. Innovators see new connections and forge new patterns. Hunches become hypotheses. Experiments are designed and conducted. Insights are gained, papers published and further conversations stoked resulting in a new cycle of discoveries.

CLARITY is one such discovery. Because most tissues are not transparent, and imaging processes are limited by light scattering; imaging deeply within tissues historically has been problematic. But with the complete removal of lipids during tissue clearing, immunostaining depth and quality improves. And because a CLARITY tissue-cleared sample remains intact, multiple rounds of fluorescent labeling could be possible. Whole tissue samples become reusable. That is helpful because who among us has only one scientific question, only one biomarker of interest?

Next Generation Immunohistochemistry staining of Tonsil Cells with classification and Segmentation

Classified and Segmented Tonsil Cells

Immunohistochemistry Staining

Next Generation Image Analysis
Another innovation that Dr. Coons could only have dreamed of are the advances in computing, specifically in machine learning, artificial intelligence, and image analysis. It is now possible to gather immunofluorescent data from multiple rounds of tissue staining and then individually segment and classify cells. A world of data is useless unless you can visualize it, process it, interpret, and understand complex relationships. Biological tissues are complex and intrinsically three-dimensional. When researchers can view spatial data in X, Y, and Z dimensions the understanding of the tissue microenvironment can spark new clinical discoveries. This is a capability we are developing at ClearLight Biotechnologies. Imagine this capability for your next experiment.

CLARITY Processed and 3D IHC Stained Breast Tissue

 

What Will You Discover?

See More Biology with Multiplex IHC in 3D

Medicine and science continue to evolve. CLARITY tissue-cleared samples combined with 3D immunohistochemistry and Tru3D Alpha* tissue analysis gives researchers more data and more ways to envision that data.

These innovations empower scientific and medical discovery. Researchers can conceive of complex experiments, make new discoveries and invent better biomarkers that are diagnostic, prognostic and predictive of disease response.

Whether your revolution is in cancer research, and more specifically immuno-oncology research and development, neurodegenerative disease or infectious diseases, you owe it to yourself to work with our technology and the scientific team that is developing it.

See More Biology and Explore Further.

Get in touch with us when you want to see more biology and explore further. Your research deserves it.
Future patients deserve it.

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Take this with you.

Analysis can be performed for select biomarkers, please contact us to find out if your target of interest is one of them. Imaging is currently performed on select fields of view instead of the entire sample.

Submission Guidelines

Tissue Sample Requirements: