Tissue Clearing Applications
Human Tonsil
Learn More About Tissue Clearing
- Tissue Clearing Methods Compared
- CLARITY Tissue Clearing Primer
- CLARITY Breast Cancer Study
- Moving Beyond Slices and Slides
Supporting Your Research Application
Tissue Clearing Tonsil
ClearLight has studied hyperplasia tonsil tissue using the CLARITY method, immunolabeling, and proprietary AI-based software on 3D volumes of tissue.
Tissue Clearing and Immunolabeling Human Tonsil
An inflamed hyperplastic tonsil tissue immunostained to identify epithelial cells (pan-cytokeratin), proliferating cells (Ki-67), and T cells (CD3). DAPI, the nuclear counterstain, marks all cells present in the tissue.
An inflamed hyperplastic tonsil tissue immunostained to identify epithelial cells (pan-cytokeratin), an immune checkpoint marker (PD-L1), and cytotoxic T cells (CD8). DAPI, the nuclear counterstain, marks all cells present in the tissue.
An inflamed human tonsil tissue stained with DAPI to clearly identify the individual cell nuclei present within the tissue. Both alpha smooth muscle actin (α-SMA) and CD31 were used to observe possible vasculature with some signal co-localization observed in certain vessels. CD45RO was used as a marker to aid in the identification of regulatory T cells (Tregs).
An inflamed human tonsil tissue that has been immunostained with pan-cytokeratin to denote the epithelial cells. The tissue was also stained with CD31, primarily an endothelial cell marker, and CD68, a marker for cells with a macrophage lineage. Finally, the nuclear stain, DAPI, is shown to demonstrate all cells present in the tonsil tissue.
An inflamed human tonsil tissue stained with DAPI to clearly identify the individual cell nuclei present within the tissue. Both alpha smooth muscle actin (α-SMA) and CD31 were used to observe possible vasculature with some signal co-localization observed in certain vessels. CD20 is utilized as a pan-B cell marker and used to identify B cells, and in rare cases can be found on some T cells.
3D Rendering
Segmentation and classification of tonsil tissue prepared using the CLARITY method and analyzed using proprietary AI-based software on 3D volumes of tissue.
This 3D software workflow is Raw Data → Cell Segmentation → Classification → Analysis.
Try tissue clearing on your own.
If you want to apply CLARITY to the tissues highlighted on this page, you will need to use the ClearLight HIGH Lipid Tissue Clearing Kit.
Tru3D® Image Analysis Software is in Development.
Subscribe to the Creating Cures Newsletter to stay informed.
Leverage our Internal R & D
When you work with us you gain the benefit of our internal experiments, assessments, and rigorous testing. In addition to performing our lab services: CLARITY Tissue Clearing, 3D IHC, and Tru3D Tissue Analysis, ClearLight scientists perform internal Research and Development to advance our understanding and applications of CLARITY to other tissue types and downstream applications. Researchers who work with us benefit from this exploratory work. The ClearLight “lab basement” is full of failed or pivoted experiments that helped inform our path to arrive at what does work. Work with us to avoid making the same mistakes that we have made or going down the blind alleys that we’ve pivoted away from.
“If you don’t want to spend the time figuring it out, we can help. We work hard to make this look easy.” - Dr. Sharla White, VP of Research and Development
Ready to Discuss Your Research Question? Meet with our Application Scientist.