Tissue Clearing Applications

Human Tonsil

Tissue Clearing Tonsil

ClearLight has studied hyperplasia tonsil tissue using the CLARITY method, immunolabeling, and proprietary AI-based software on 3D volumes of tissue.

Tissue Clearing and Immunolabeling Human Tonsil

An inflamed hyperplastic tonsil tissue immunostained to identify epithelial cells (pan-cytokeratin), proliferating cells (Ki-67), and T cells (CD3). DAPI, the nuclear counterstain, marks all cells present in the tissue.

An inflamed hyperplastic tonsil tissue immunostained to identify epithelial cells (pan-cytokeratin), an immune checkpoint marker (PD-L1), and cytotoxic T cells (CD8). DAPI, the nuclear counterstain, marks all cells present in the tissue.

An inflamed human tonsil tissue stained with DAPI to clearly identify the individual cell nuclei present within the tissue. Both alpha smooth muscle actin (α-SMA) and CD31 were used to observe possible vasculature with some signal co-localization observed in certain vessels. CD45RO was used as a marker to aid in the identification of regulatory T cells (Tregs).

 

An inflamed human tonsil tissue that has been immunostained with pan-cytokeratin to denote the epithelial cells. The tissue was also stained with CD31, primarily an endothelial cell marker, and CD68, a marker for cells with a macrophage lineage. Finally, the nuclear stain, DAPI, is shown to demonstrate all cells present in the tonsil tissue.

An inflamed human tonsil tissue stained with DAPI to clearly identify the individual cell nuclei present within the tissue. Both alpha smooth muscle actin (α-SMA) and CD31 were used to observe possible vasculature with some signal co-localization observed in certain vessels. CD20 is utilized as a pan-B cell marker and used to identify B cells, and in rare cases can be found on some T cells.

3D Rendering

Segmentation and classification of tonsil tissue prepared using the CLARITY method and analyzed using proprietary AI-based software on 3D volumes of tissue.
This 3D software workflow is Raw Data → Cell Segmentation → Classification → Analysis.

Tru3D® Image Analysis Software is in Development.

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 Collaborative Research Organization for Tissue Clearing Tonsil and Tru3D Tissue Analysis

Leverage our Internal R & D

When you work with us you gain the benefit of our internal experiments, assessments, and rigorous testing. In addition to performing our lab services: CLARITY Tissue Clearing, 3D IHC, and Tru3D Tissue Analysis, ClearLight scientists perform internal Research and Development to advance our understanding and applications of CLARITY to other tissue types and downstream applications. Researchers who work with us benefit from this exploratory work. The ClearLight “lab basement” is full of failed or pivoted experiments that helped inform our path to arrive at what does work. Work with us to avoid making the same mistakes that we have made or going down the blind alleys that we’ve pivoted away from.

“If you don’t want to spend the time figuring it out, we can help. We work hard to make this look easy.” - Dr. Sharla White, VP of Research and Development

Ready to Discuss Your Research Question? Meet with our Application Scientist.

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Analysis can be performed for select biomarkers, please contact us to find out if your target of interest is one of them. Imaging is currently performed on select fields of view instead of the entire sample.

Submission Guidelines

Tissue Sample Requirements: