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Immunostaining Buffer

$100.00

This reagent has been optimized for the immunostaining of soft tissues cleared using the CLARITY Tissue Clearing Kit (sold separately). After lipid clearing, the tissue-hydrogel hybrid is both transparent to light and permeable to molecules, enabling immunostaining and imaging. This kit is intended for Research Use Only.

Having issues with immunostaining or antibody feasibility?  Visit CLARITY 101 to learn more or contact Lab Services.

Kit Contents: Immunostaining Buffer (105 mL), Procedures and Guidance Insert

13 in stock (can be backordered)

SKU: TCR-IB Categories: , Tags: ,

Description

This optimized reagent for immunostaining is used for soft tissues cleared using the CLARITY Tissue Clearing Kit - High Lipid or Low Lipid. CLARITY is a tissue clearing method used to render tissues optically transparent, allowing for non-destructive tissue processing and 3D imaging of both normal and diseased soft tissues. In the CLARITY tissue clearing method, the tissue is first embedded with hydrogel monomers to create a matrix scaffold, then the light scattering lipids are removed. After lipid clearing, the tissue-hydrogel hybrid is both transparent to light and permeable to molecules, enabling immunostaining and imaging using a confocal or light-sheet microscope.

The optimized reagent for immunostaining supports step 4 as shown in the Tissue Processing Workflow diagram below.

New to CLARITY tissue clearing? Visit CLARITY 101 to learn more. Short on time or capacity? Consider ClearLight’s Lab Services. See 3D imaging of normal and diseased tissues on ClearLight's YouTube Channel.

ClearLight Biotechnologies Tissue Processing Workflow - CLARITY Tissue Clearing Kit

Applicable Tissues for Kit Type
High Lipid Kit Low Lipid Kit
Embryo Lung Tissue
Brain Tissue Kidney Tissue
Lymph Nodes Skin Samples
Calcified Tissues (Bone) Cancer (Breast, Lung, etc)
Tonsil Pancreas
Organoids Liver
Eye Intestine
Cell Pellet Muscle
Spinal Cord Heart
Spleen
Bladder

Additional information

Weight .25 lbs

SDS - Immunostaining

Download

Safety Data Sheet for Immunostaining Buffer

Analysis can be performed for select biomarkers, please contact us to find out if your target of interest is one of them. Imaging is currently performed on select fields of view instead of the entire sample.

Submission Guidelines

  • The submitted sample must be pre-fixed in either
    • 4% paraformaldehyde (PFA) or
    • 10% neutral buffered formalin (NBF) for 24 hours.
      • Unfixed samples will not be accepted for lab services.
      • Samples prepared with fixatives other than 4% PFA or 10% NBF will not be accepted and have not been validated with our protocol.
    • The tissue must be noted:
      • perfused or not perfused
    • All submitted samples must meet the noted specifications listed in Tissue Sample Requirement.
      • Samples that do not meet the size, species, or tissue sample type will not be accepted.
    • Samples must be shipped in a sealed container (pick 1)
      • PBS or TBS solution.
    • Shipped samples should be scheduled for delivery to ClearLight Biotechnologies Monday through Wednesday with a provided tracking number.
      • Shipping company
      • Tracking number

Tissue Sample Requirements:

    • Sample Size dimensions:
      • 5 mm dia. x 1 mm thick or
      • 5 x 5 x 1 mm
    • Acceptable Sample Species:
      • Mouse
      • Human
    • Acceptable Tissue type:
      • Tumor
        • Syngeneic
        • Xenograft
        • PDX (patient derived xenograft)
        • Primary
      • Kidney
      • Spleen
      • Lung
      • Tonsil
      • Brain
      • Pancreas
      • Liver
      • Mammary