Immunostaining Buffer

$100.00

This reagent has been optimized for the immunostaining of soft tissues cleared using the CLARITY Tissue Clearing Kit (sold separately). After lipid clearing, the tissue-hydrogel hybrid is both transparent to light and permeable to molecules, enabling immunostaining and imaging. This kit is intended for Research Use Only.

Having issues with immunostaining or antibody feasibility?  Visit CLARITY 101 to learn more or contact Lab Services.

Kit Contents: Immunostaining Buffer (105 mL), Procedures and Guidance Insert

SKU: TCR-IB Category: Tags: ,

Description

This optimized reagent for immunostaining is used for soft tissues cleared using the CLARITY Tissue Clearing Kit – High Lipid or Low Lipid. CLARITY is a tissue clearing method used to render tissues optically transparent, allowing for non-destructive tissue processing and 3D imaging of both normal and diseased soft tissues. In the CLARITY tissue clearing method, the tissue is first embedded with hydrogel monomers to create a matrix scaffold, then the light scattering lipids are removed. After lipid clearing, the tissue-hydrogel hybrid is both transparent to light and permeable to molecules, enabling immunostaining and imaging using a confocal or light-sheet microscope.

The optimized reagent for immunostaining supports step 4 as shown in the Tissue Processing Workflow diagram below.

New to CLARITY tissue clearing? Visit CLARITY 101 to learn more. Short on time or capacity? Consider ClearLight’s Lab Services. See 3D imaging of normal and diseased tissues on ClearLight’s YouTube Channel.

ClearLight Biotechnologies Tissue Processing Workflow - CLARITY Tissue Clearing Kit

Additional information

Weight .25 lbs

Analysis can be performed for select biomarkers, please contact us to find out if your target of interest is one of them. Imaging is currently performed on select fields of view instead of the entire sample.

Submission Guidelines

Tissue Sample Requirements: