Description
This optimized reagent for immunostaining is used for soft tissues cleared using the CLARITY Tissue Clearing Kit - High Lipid or Low Lipid. CLARITY is a tissue clearing method used to render tissues optically transparent, allowing for non-destructive tissue processing and 3D imaging of both normal and diseased soft tissues. In the CLARITY tissue clearing method, the tissue is first embedded with hydrogel monomers to create a matrix scaffold, then the light scattering lipids are removed. After lipid clearing, the tissue-hydrogel hybrid is both transparent to light and permeable to molecules, enabling immunostaining and imaging using a confocal or light-sheet microscope.
The optimized reagent for immunostaining supports step 4 as shown in the Tissue Processing Workflow diagram below.
New to CLARITY tissue clearing? Visit CLARITY 101 to learn more. Short on time or capacity? Consider ClearLight’s Lab Services. See 3D imaging of normal and diseased tissues on ClearLight's YouTube Channel.
Additional information
| Weight | .25 lbs |
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