What is ClearLight’s immunostaining workflow?

Analysis can be performed for select biomarkers, please contact us to find out if your target of interest is one of them. Imaging is currently performed on select fields of view instead of the entire sample.

Submission Guidelines

• The submitted sample must be pre-fixed in either
  • 4% paraformaldehyde (PFA) or
  • 10% neutral buffered formalin (NBF) for 24 hours.
    • Unfixed samples will not be accepted for lab services.
    • Samples prepared with fixatives other than 4% PFA or 10% NBF will not be accepted and have not been validated with our protocol.
  • The tissue must be noted:
    • perfused or not perfused
  • All submitted samples must meet the noted specifications listed in Tissue Sample Requirement.
    • Samples that do not meet the size, species, or tissue sample type will not be accepted.
  • Samples must be shipped in a sealed container (pick 1)
    • PBS or TBS solution.
  • Shipped samples should be scheduled for delivery to ClearLight Biotechnologies Monday through Wednesday with a provided tracking number.
    • Shipping company
    • Tracking number

Tissue Sample Requirements:

• • Sample Size dimensions:
    • 5 mm dia. x 1 mm thick or
    • 5 x 5 x 1 mm
  • Acceptable Sample Species:
    • Mouse
    • Human
  • Acceptable Tissue type:
    • Tumor
      • Syngeneic
      • Xenograft
      • PDX (patient derived xenograft)
      • Primary
    • Kidney
    • Spleen
    • Lung
    • Tonsil
    • Brain
    • Pancreas
    • Liver
    • Mammary